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KMID : 0380020120270010033
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Korean Journal of Biotechnology and Bioengineering
2012 Volume.27 No. 1 p.33 ~ p.39
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Identification of Curcuminoids from Turmeric (Curcuma longa) Using Ultrasonic Wave and Dipping Method
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Lee Kwang-Jin
Ma Jin-Yeul
Kim Young-Sik
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Abstract
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In this study three major curcuminoids in turmeric curcumin (1), demethoxycurcumin (2) and bismethoxycurcumin (3) were efficiently extracted by optimizing extraction condition and simultaneously identified using a fast and reliable RPHPLC-UV-MS and TLC method. The analysis by the C18 column was performed and the UV wavelength was fixed at 425 nm. In this result, the total extraction yield of turmeric (Curcuma longa) was increased with extraction time from 1 to 7 h. So, optimum extraction time is 4 h. Also, the highest yield of extraction amount 0.433g 8.66% was obtained by ultrasonic waves with quarter frequency kHz and an extraction time of 7 h. The experiment method was consistent with theoretical Value r2 = 0.987 (1), 0.997 (2) and 0.998 (3). Moreover, LC-MS analysis provided efficiently molecular weight information of three major curcuminoids in turmeric extracts and high purity (~95%) of the curcuminoids were obtained. This work offers would be useful for chemical and biological studies of natural plants and its products.
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KEYWORD
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Analysis, Curcuma longa, Curcuminoids, HPLC, Separation, Isolation
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